Tet B or not tet B: Advances in tetracycline-inducible gene expression
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چکیده
منابع مشابه
Tet B or not tet B: advances in tetracycline-inducible gene expression.
Be it the B class, or another class of tetracycline (tet) repressor, the utility and specificity of transcriptional regulators based on this family of prokaryotic DNA binding proteins is unparalleled. A method for regulating gene expression at will in mammalian cells has long been the holy grail. Transfections of uncontrolled numbers of plasmids and unregulated gene expression were breakthrough...
متن کاملPlasmid-encoded Tet B tetracycline resistance in Haemophilus parasuis.
The complete sequence of two plasmids, pHS-Tet (5.1 kb) and pHS-Rec (9.5 kb), isolated from Haemophilus parasuis strain HS1543 has been obtained. Plasmid pHS-Tet contains four open reading frames including a tet(B) tetracycline resistance gene which unusually did not have an associated tetR repressor gene. From a total of 45 H. parasuis isolates surveyed (15 international reference strains, 15 ...
متن کاملCharacterization of tetracycline-inducible bitransgenic Krt12rtTA/+/tet-O-LacZ mice.
PURPOSE To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline. METHODS A gene-targeting construct containing an internal ribosomal entry site-reverse tetracycline transcription activator (IRES-rtTA) cassette was inserted into the Krt12 allele (keratin 12 gene) to produce a knock-in Krt12(rtTA/+) mouse line ...
متن کاملTet-On Systems For Doxycycline-inducible Gene Expression
The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic biological research to biotechnology and gene therapy applications. These systems are based on regulatory elements that control the activity of the tetracycline-resistance operon in bacteria. The Tet-Off system allows silen...
متن کاملEvaluation of resistance genes in Escherichia coli isolated from toilets in the Islamic Azad University, Mashhad and genome sequencing (tet B, tet A, tet R, OXA 10 and OXA 48) in tetracycline-resistant strains
Aims and Background: E. coli is a normal flora of the human and animal intestine that sometimes causes pathogenesis in the intestine and urinary tract. The aim of this study is to investigate resistance genes in E. coli isolated from toilets in the Islamic Azad University of Mashhad and perform genome sequencing in tetracycline-resistant strains. Methods: This cross-sectional study was perform...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1999
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.96.3.797